Viability of spheroid on NCP is better than that on the low attachment-type plate

Spheroids on NCP show high viability due to attaching on the culture surface. Following pictures show dead cells inside spheroids stained with EthD-1 (Invitrogen).

Cell proliferation of NCP spheroid

The growth of cells in spheroids cultured on low attachment-type plate is usually slow compared to monolayer cultured cells. [Ref. Lpn.l.Cancer Res.89,p110-115(1998)]. However, spheroids cultured on NCP show the growth to a similar extent as monolayer.

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High cell viability inside spheroid on NCP

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Advantage of NCP

No matrix is containing (micro-pattern mimics matrix on NCP)

→Manufacture of the pattern is precisely controlled so there is no lot-to-lot variation.
→No limitation of assays and easy to collect cells

Low intrinsic fluorescence

→Easy in observation

Stay attached on the culture surface

→High cell viability
→Grow to a similar extent as monolayer
→Can be used for confocal microscopy