Each cell line manifests the cell's properties to take the characteristic form.
＜Evaluation example＞
For example, anticancer agents, which are effective on animals but ineffective on monolayer-cultivated cells, have been confirmed to show similar reaction as in vivo to spheroid-cultivated cells.
(This is the example of anticancer agents used clinically.)
Anticancer
agent 1
Anticancer
agent2
Monolayer
cultivation
＋＋＋
＋
Animal
Test
＋
＋＋＋
3-D cultivation （by NCP）
＋
＋＋＋
Attention!
By starting the NCP treatment after shperoid formation, the anticancer drug shows different effects.
Trial Kit for Established Cell Line (product No.NCL-3)
This kit enables you to choose the spheroid formation condition for the cell line (of various kinds including cancer cells) in your lab. Even the same cell line can have different optimal pattern/medium according to the passage number or preservation condition of the cell.
The media in your lab are basically usable. Use our medium as the reference.
Kit content:
Volume/Number
Preservation condition
Validity period
Plate (2 patterns)
96well plate each×1
Room temp
3 months
Media (2 kinds)
10ml each ×１
Cool storage （4－8℃）
3 months
Spheroid lysis
1.5ml ×2
Room temp
3 months
* The cell number measurement kit (sold on the market) is not included.
