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Cell Cultivation Top Page ＞FAQs
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| Q.01→How does the Spheroid cultivation differ from the usual monolayer cultivation? |
| Q.02→Why is the Spheroid created? |
| Q.03→What cell species have you succeeded in cultivating through Spheroid cultivation? |
| Q.04→What is the array of commercially available SCIVAX plate products? |
| Q.05→Would you tell me the cultivaton protocol? |
| Q.06→Are the cultivated cells on offer? |
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| Q.01?Are the assay systems involving the medium replacement available? |
| Q.02?You mention the per cell albumin product. How do you determine the cell number? |
| Q.03?How long does the spheroid formation take after seeding the cells? |
| Q.04?What does it mean that the spheroid-forming ability is low? |
| Q.05?How do you confirm apoptosis? |
| Q.06→Of the cells you have so far tried, what percentage have succeeded in forming spheroids? |
| Q.07→Are cell dispersion and cell lysis possible after spheroid formation? |
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| Q.01?What are the differences between the 3 patterns SCIVAX provides? |
| Q.02→Who are the distributors of your plates? |
| Q.03?What treatment is applied on the plate surface? |
| Q.04?Do your plates conform to the SBS standard? |
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Q.01 How does the Spheroid cultivation differ from the usual monolayer cultivation? |
The spheroid, which characteristically achieves the three-dimensional form, will display properties closer to the living organism than the monolayer cells, for example on liver cells.
For more details, click here.
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Q.02 Why is the Spheroid created? |
When being cultivated on the finely-structured plate, which will provide the foothold to the cells, the 3-D spheroid can be formed.
For more details, click here.
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Q.03 What cell species have you succeeded in cultivating through Spheroid cultivation? |
Various censer cells, as well as various normal cells.
For more details, click here.
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Q.04 What is the array of commercially available SCIVAX plate products? |
We reccomend different products according to the cell species. Currently, as an initial sales offering, we offer as a set the plate (96Well) spheroid culture medium and the spheroid lysis buffer, with which you can try three patterns.
For more details, click here.
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Q.05 Would you tell me the cultivaton protocol? |
| We recommend protocols for each cell species based on our past records. For more details, referto the instruction manual.
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Q.06 Are the cultivated cells on offer? |
| Adipocytes and chondrocytes are available. We will accommodate your requests as much as possible. For more details, please contact us by e-mail.
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Q.01 Are the assay systems involving the medium replacement available? |
| Yes, they are. The spheroid cultivation which allows for the medium replacement (i.e.adhesiveness)
is the feature of the NCP.
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Q.02 You mention the per cell albumin product. How do you determine the cell number? |
| We measure the ATP density or the DNA amount and convert the value to the cell number. To measure the cell number correctly, the complete spheroid dissolution is necessary. Please use the proprietary solution.
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Q.03 How long does the spheroid formation take after seeding the cells? |
It depends on the cell. For example, for the cancer cell it takes about 2 to 5 days.
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Q.04 What does it mean that the spheroid-forming ability is low? |
| It means that the cell does not form the 3-D structure easily. It does not mean that the spheroid is not formed at all.
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Q05 How do you confirm apoptosis? |
| Through an ethidium bromide/acridine orange staining.
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Q.06 Of the cells you have so far tried, what percentage have succeeded in forming spheroids? |
| By optimizing the pattern and the cultivation medium, almost 100% of the cancer cells have been successfully cultivated. As for the primary culture, the spheroid cultivation is possible by using the NCP-prim kit with optimized adhesiveness.
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Q.07 Are cell dispersion and cell lysis possible after spheroid formation? |
They are possible. For more details, click here.
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Q.01 What are the differences between the 3 patterns SCIVAX provides? |
The size and the form are different. As the different cell species can have different optimal patterns, we have tested about 10 patterns in selecting these three versatile patterns.
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Q.02 Who are the distributors of your plates? |
We sell the trial plates directly to our customers after giving them technological explanation of details inculuding cultivation conditioning.
We will consider using a proposed distributor on request.
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Q03 What treatment is applied on the plate surface? |
The plate surface has been subjected to treatments so that customers can perform spheroid cultivation basically without any treatments such as various kinds of coating.
For primary culture or other cultivation procedures, we supply instruction in our recommended methods (using NCR-prim and NCM-prim).
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Q04 Do your plates conform to the SBS standard?
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Yes, they do. They can be used for various instruments and also for the assay systems.
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